menonton indahnya kulit

Utilization of gene silencing effectors, such as microRNA (miRNA) and small airpin RNA (shRNA), provides a powerful new strategy for human skin care in vivo, particularly for hyperpigmentation treatment and aging prevention. In this study, tyrosinase (Tyr), the rate-limiting enzyme of melanin (black pigment) biosynthesis, was served as a target for treatment of hyperpigmentation in mouse and human skins. There are over 54 native microRNA capable of silencing human tyrosinase for skin whitening and lightening. To this, we have designed a mir-434-5p homologue and used it to successfully demonstrate the feasibility of miRNA-mediated skin whitening and lightening in vitro and in vivo. Under the same experimental condition in the trials, Pol-II-directed intronic mir-434-5p expression did not cause any detectable sign of cytotoxicity, whereas siRNAs targeting the same sequence often induced certain nonspecific mRNA degradation as previously reported. Because the intronic miRNA-mediated gene silencing pathway is tightly regulated by multiple intracellular surveillance systems, including Pol-II transcription, RNA splicing, exosomal digestion and nonsense-mediated RNA decay (NMD), the current findings underscore the fact that intronic miRNA agents, such as manually re-designed mir-434-5p homologues, are effective, target-specific and safe to be used for skin whitening without any detectable cytotoxic effect. Given that the human skins also express a variety of other native miRNAs, we may re-design these miRNAs based on their individual functions for skin care, which may provide significant insights into areas of opportunity for new cosmetic and/or therapeutical applications.

Whitening Lotion/Cream – for whitening with anti-aging ingredient; best for overall whitening/lightening of skin.
Lightening Lotion/Cream – for whitening with stronger potency than the Whitening Lotion/Cream to target small dark spots and hyperpigmentation.

Whitening and lightening

Formula Standart			Formulasi Pembanding			Formula Modifikasi
Nama bahan	Fungsi	[ ]	Nama Bahan	Fungsi	[]	Nama Bahan	Keterangan	[]
Arlacel 165 (gliseril stearat& PEG 100stearat	Emulsifier non ionic, surfaktan (www.croda.com)	8%	Water	Solvent		gliseril monostearat; asam stearate	Arlacel 165(10%gliseril monostearate,90%PEG100 stearate ) PEG100 dibuat dengan mengkombinasikan minyak alam dengan asam stearate untuk membentuk ester larut air atau secara sintetik dapat dibuat dengan mengkombinasikan oxirane (etilen dioksida) dan asam lemak (asam stearat)	6% 3% C%t 2nd ed vol 1,p195
Crodamol CSP Cetostearil palmitat	emulsifier	4%	Buthelyn alcohol	Solvent		Cetostearil alkohol		4%
Liq base CB 3929 (mineral oil & lanolin alcohol)	Emolien, emulsifying agent, plastisizer	4%	Isonoyl isonoate	Emolien		Mineral oil; Setil alkohol	Pada formula baby o/w Cosmetic&toiletry 2nd ed, vol1, p54	5% 4%
Crodalan IDL (isopropyl lanolat)	emolien	2%	Cetyl alcohol	Emulsifying agent		Isopropil miristat		20%
Polycol 15 (ester PEG dari lanolin alcohol dengan harga etoksitat rata2x 15)	Dispersing agent, emulsifier o/w, wetting agent	3%	Lanolin alcohol			Tween 80; Span 60	HLB polychol 15=12,7	0,2% 0,3%
Setil alcohol	Emolien, emulsifying agent, stiffening agent, water absorption	1%	Soluble collagen	Menjaga elastisitas kulit		Setil alkohol	-	1%
Nipagin	pengawet	0,05%	Squalen	Skin moisturizer		Nipagin	-	0,05%
Nipasol		0,15%	Morus bombycis roat extract	Lightening / whitening antioxidant		Nipasol	-	0,15%
Propilen glikol	humektan	5%	Glyserin	Humektan		Propilen glikol	-	5%
Kelzan (xathan gum)	Viscosity increasing agent	2%	Urea	Skin moisturizer		Veegum (Mg Al Silikat)	Viscosity modifier 2–10 % (HPE, p.394)	2%
Na metabilsufit	Antioksidan	0,1%	Sacharide	Humektan		Na metabisulfit		0,1%
Asam askorbat	Antioksidan, stabilizer,	O,1%	Magnesium aspartate	Chelating agent		Asam askorbat	Lightening agent	5%
Hidrokuinon	BA pemutih kulit	2%	Glycine	Buffering agent		-	Diganti Arbutine	3,77%
Air	solvent	64,4%	Alanine	Antioksidan		Air
Parfum	Pengharum	Qs	Creatine	Skin conditioning		Parfum		Qs
			PEG 40 hydrogenated castor oil	Emolien
			Trideceth-9	Emulsifying surfaktan
			Bisabolol	Skin conditioning
			Ascorbyl dipalmitate	Antioxidant
			Panthenol	Skin conditioning
			Glyceril stearate	Emolien, emulsifying agent
			PEG 100 stearate	Emulsifying agent
			Dimethicone	Antifoaming agent, emolien (hpe5th, p586)
			Potato starch modified	Viscosity Controlling
			Allantoin	Melembutkan & melembabkan kulit (md28th,400)
			Titanium dioxide	Uv filter
Skin lightening cream, harry sp.269 Bentuk:			Alumina	Opadcifying viscocity controlling
			Silica
			Sodium polyacrylate	Emulsion stabilizing
			Cetearyl ethylher xanoate	Emolien, skin conditioning
			IPM	Emolien
			Dea-cetyl fosfat	Emulsifying, surfaktan
			Ethyl hexyl palmitate	Sunscreen agent
			Sodium lactate	Anti microbial, emulsi fying
			Ginko biloba leayes extract	Skin conditioning
			Sodium PCA	Melembabkan kulit
			Betaine	Humektan, skin conditioning
			sorbitol	Humektan
			Proline	Skin conditioning
			serine	Skin conditioning
			treonin	Antistatic
			Glutamic acid	Buffering agent
			Hydrous alsilikat	Absorben opacifying
			Lactid acid	Acidifying agent
			Aluminium	Absorben, anti  caking
			Starch octenyl succinate	Viscosity controlling
			Almond oil	Emolien, oleaginous vehicle
			Tocopherol acetate	Antioksidan
			Glikoprotein	Nutrisi kulit
			Retynyl palmitate	Melembutkan & melembabkan kulit
			Xanthan gum	Viscosity increasing agent
			Phenoxy alkohol	antimicrobial
			Metyl aparaben
			Ethyl paraben	Pengawet
			Propyl paraben
			Butyl paraben
			Parfume	Pengharum
			Bentuk: krim o/w Biokos Night radiance cream

Bentuk sediaan dasar

a.       Bentuk:krim o/w dan w/o

Krim adalah padatan atau emulsi semipadat, dan dapat diaplikasikan untuk produk non cairan seperti wax-solvent-based mascaras,liquid eyeshedow dan ointments

(harry’s cosmeticology,P50)

Syarat umum krim

-kandungan minyak sedang

-kadang sangat berminyak tetapi mudah menyebar

-bentuk w/o atau o/w

b.      Bentuk sediaan terpilih

Bentuk:krim pemtih dan pencerah

Krim pemutih adalah sediaan yang digunakan dengan 2 jalur untuk mencerahkan warna kulit dengan mereduksi pigmentasi: menghilangkan warna melamin /warna gelap dan mencegah pembentukan melamin (harry”s and cosmeticology p 266)

Syarat umum (harrys cosmeticology ,P50)

-kandungan minyak lebih banyak agar dapat menembus stratum basale

(tempat target obat)

PH netral

-mengandung surfaktan u/ meningkatkan penetrasi

-ph netral

-mengandung surfaktan u/meningkatkan penetrasi

- mudah menyebar

Cara pembuatan

1.       Kembangkan Veegum dalam air panas

2.       Larutkan nipagin nipasol dalam propilen glikol

3.       Fase minyak: cetostaryl alcohol, cetyl alcohol, gliseril monostearat, as stearat, sebagian mineral oil

4.       No 1,2,3 masukkan mortar panas

5.       Arbutin levigasi dengan campuran tween 80 dan span 60

6.       No5 di + campuran vit c+ air

7.       No6+campuran Na metabisulfit + air

8.       No & masukkan dalam no $ aduk ad terbentuk krim